This Waters Alliance e2695 HPLC system comes with Waters 2489 UV/Vis Detector. They are in great condition and have been tested at SpectraLab Scientific.
Specifications:
Solvent Management | |
Number of solvents | One to four |
Solvent conditioning | Vacuum degas, two operating modes, four chambers, 500 µL internal volume per chamber |
Flow rate range | 0.000 to 10.000 mL/min (0.050 to 5.000 mL/min typical) in 0.001 mL/min increments |
Compressibility compensation | Automatic continuous |
System delay volume | < 650 µL, independent of backpressure at 1 mL/min |
Plunger seal wash | Integral, active, programmable |
Gradient profiles | 11 gradient curves (including linear, step [2], concave [4], and convex [4]) |
Dry prime/ wet prime | Automatic front panel control, SystemPREP function for automatic solvent(s) purge |
Flow ramping | Time (0.01 to 30.00 min in 0.01 min increments) to reach maximum flow rate |
Maximum operating pressure | 5000 psi (345 bar) (0.010 to 3.000 mL/min) programmable upper and lower limits |
Composition range | 0.0% to 100.0%, in 0.1% increments |
Composition accuracy | +0.5% absolute, independent of backpressure (proportioning valve pair test,[degassed methanol: methanol/proplyparaben, 2.0 mL/min, 254nm]) |
Composition precision | <0.15% RSD or <0.02 min SD, six replicates, based on retention time or volumetric measures (0.200 to 5.000 mL/min),isocratic premix |
Flow accuracy | +1% or 10 µL/min, which is greater, 0.200 to 5.000 mL/min, |
Sample management | |
Number of samples vials | 120 vials, configured in five carousels of 24 vials each |
Number of sample injections | 1 to 99 injections per sample vial |
Sample delivery precision | Typically <0.5% RSD, 5 to 80 µL (degassed methanol: water 60:40 dial-a-mix, 1 mL/min, six replicates, phenone mix, 254 nm) |
Sample carryover | <0.01% for caffeine, under specified conditions |
Injection needle wash | Integral, active, programmable |
Injection accuracy | +1µL (+2%)(50µL, N-6), sample: 100% degassed water, analytical solvent: 100% degassed methanol |
Standard sample vial | 2 mL |
Advanced operations | Priority samples, auto additions, auto standards |
Injection volume range | 0.1 to 100 µL, standard; 0.1 to 2000 µL, with optional sample loop |
Injector linearity | >0.999 coefficient of deviation (1.000 to 100.000 µL) |
Minimum sample required | 10 µL, using low volume inserts |
Sample temperature control (optional) | 4 to 40°C, programmable in 1°C increments |
Column heater (optional) | 20 to 65 °C, in 1 °C increments (5°C above ambient) |
Column heater/cooler(optional) | Ambient minus 15°C to 4°C (whichever is greatest) up to 65°C, in 1°C increments |
Waters 2489 UV/Vis detector
Condition | Specification |
Wavelength range | 190 to 700 nm |
Bandwidth | <5 nm |
Wavelength accuracy | +1.0 nm |
Wavelength repeatability | +0.1 nm |
Single λ dry noise | <5 µAU (at 230 nm, 1 –second digital filter, 30-second segments, 10-Hz data rate, analytical cell) |
Wet noise | <8 µAU (at 230 nm, 1-second digital filter 30-second segments, 10-Hz data rate, analytical cell) |
Dual channel noise (dry) | <35 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; dry, analytical cell) |
Dual channel noise (wet) | <45 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; 1 ml/min, flow of acetonitrile, analytical cell) |
Linearity | <5% at 2.5 AU, propylparaben at 257 nm |
Drift | 1.0 x 10-4 AU/hr. max (∆T = +2°C/hr.) |
Thermal drift | 1.0 x 10-4 AU/°C max (∆T = +2°C/hr.) |
Sensitivity setting range | 0.0001 to 4.0000 AUFS |
Filter setting range | Single-wavelength: 0.1 to 5.0 seconds Dual-wavelength: 1 to 50 seconds |
Time-constant | To 5.0-second Hamming filter |
Digital data rates | 1,2,5,10,20,40,80 Hz (single channel) 1,2 Hz (dual channel) |
Analog output data rates (single λ mode) | 10,20,40,80 Hz (channel A) 10 Hz only (channel B) |
Lamp source | 30-W High Brightness Deuterium lamp, 0.5 nm aperture, pre-aligned 2000 silicon photodiodes (matched pair) |
Second-order filter | Automatic for wavelengths > 370 nm |
Wavelength calibration filter | Erbium filter, used at startup or on demand |
Flow cell | TaperSlit flow cell design |
Nitrogen purge | Purge fitting present on optics bench |
Path length | 10 mm (standard analytical) |
Cell volume | 10 µL (standard analytical) |
Pressure limit | 6895 kPa (69 bar,1000 psi) |
Materials | 316 stainless steel, fused silica, Tygon |
Operating temperature | 4 to 40 °C (39 to 104 °F) |
Operating humidity | 20% to <95%, noncondensing |
Shipping and storage | -30 to +60 °C |
Shipping and storage humidity | 0% to <95% noncondensing |