This Agilent 1200 Prep HPLC includes:
- Agilent 1200 Series Preparative Pump G1361A -QTY 2
- Agilent 1200 Series Fraction Collectors G1364B
- Agilent 1200 Series Dual Loop Autosampler (G2258A) or Agilent 1200 Series Preparative Autosampler (G2260A)
- Agilent 1200 Series G1315B DAD
Features:
- Dedicated systems with low delay volumes optimized for high recovery and purity and desired throughput
- Software solutions for different user needs allows you to configure your own purification system
- Fraction collection based on time, peak, and/or masses (or even manually) allows you to collect only the desired fractions
- Automated delay volume calibration for easy and fast system set-up
This Agilent 1200 Prep HPLC is in great condition. It has been tested at SpectraLab Scientific. A 90-day warranty is included.
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Performance Specifications:
| Agilent 1200 Series Preparative Pump G1361A | |
|---|---|
| Type | Specification |
| Hydraulic system | Dual pistons in parallel |
| Flow range Settable | 0.001 – 100 ml/min |
| Flow precision | <0.5% RSD |
| Pressure range | 20 to 400 bar (5880 psi) system pressure |
| Compressibility compensation | User-selectable, based on mobile phase compressibility |
| Recommended pH range | 1.0 – 12.5, solvents with pH <2.3 should not contain acids which attack stainless steel |
| Control and data evaluation | Agilent ChemStation for LC and LC/MS |
| Communication | Controller-area network (CAN), RS-232, APG Remote: ready, start, stop and shut down signals, CAN-DC OUT, LAN optional |
|
Safety and maintenance |
Extensive diagnostics, error detection and display (through control
module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. |
| GLP features |
Early maintenance feedback (EMF) for continuous tracking of
instrument usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors. |
| Housing | All materials recyclable |
| Agilent 1200 series Fraction Collectors G1364B | |
|---|---|
| Type | Specification |
| trigger modes |
Time slices,
Peak (threshold, up- / downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323B Control Module) Agilent 1200 Series DAD/MWD detectors (G1315A/B/C, G1365 A/B/C), the Agilent 1200 Series fluorescence detector and the Agilent G1946C/D, G1956A/B LC-MSD are fully supported other detectors can be used but are not supported for fraction collection. |
| operating modes |
Discrete fractions: default mode for all vessels. The flow is diverted to
waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste |
| Fraction capacities and trays |
4 x well-plates full tray (MTP)*
(for use with deep well plates, only) 2 × well-plates std. tray (MTP) (for use with deep well plates, only) + 10 × 2 ml vials* (+ 1 half tray) 100 x 2 ml in std. tray (+ 1 half tray)* 3 x 40 x 2 ml in half tray* 3 x 15 x 6 ml in half tray* Full tray with 40 test tubes (30 mm OD, max. height 100 mm, ~45 ml / tube) Full tray with 60 test tubes (25 mm OD, max. height 100 mm, ~25 ml / tube) Full tray with 126 test tubes (16 mm OD, max. height 100 mm, ~12 ml / tube) Full tray with 215 test tubes (12 mm OD, max. height 100 mm, ~7 ml / tube) Installed trays are automatically detected and identified. For the with uncapped vials, tests tubes and well plates, only! |
| test tube / plate sizes | Minimum 48 mm to 100 mm maximum |
| Maximum tube Volume | ca. 45 ml |
| Maximum flow rate | 100 ml/min (depending on viscosity and generated back pressure, max, 6 bar at the diverter valve) |
| Delay volumes [µl] |
Fraction collector inlet to diverter valve: ~500 (typical, depends on length of
the tubing) Diverter valve: ~15 Diverter valve to needle: ~110 Needle: ~5 |
| Delay calibration sensor |
Single wavelength absorbance detector working at 654 nm, consisting of a
LED and a photo diode |
| Diverter valve |
3/2 Diverter valve with low internal volume (15 μl), switching time
< 100 ms, maximum operating pressure 6 bar |
| cooling |
Optional (with additional G1330B), performance depending on ambient
conditions and the volume of collected fractions |
| maximum capacity |
3 fraction collectors in parallel plus one recovery fraction collector
connected via 12-Position, 13-Port Selector valve (PN G1160A |
| GLP features |
Early maintenance feedback (EMF), electronic records of maintenance and
errors |
| Interfaces |
Controller-area network (CAN).
optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves |
| Safety features |
Leak detection and safe leak handling, error detection and display, exhaust
fan for fume extraction of hazardous vapors |
| Agilent 1200 Series DualLoop Autosampler G2258A | |
|---|---|
| Type | Specification |
| GLP features |
Early maintenance feedback (EMF), electronic records of maintenance and
errors |
| Communications |
Controller-area network (CAN). RS232C, APG-remote standard,
optional: four external contact closures and BCD vial number output optional: LAN communication card |
| Instrument control |
Agilent ChemStation software
Agilent HT Purification SW Agilent G1323B Control Module |
| Safety features |
Leak detection and safe leak handling, low voltages in maintenance areas,
error detection and display |
| Injection modes |
Partial loop filling
Complete loop filling |
| Injection range |
0.1 – 5000 µl in 0.1 µl increments
Recommended injection range 10 – 5000 µl |
| Area precision Complete Loop Fill | Typically < 0.5% RSD,(50 µl loop, overfill factor 3 or greater) |
| Area precision Partial Loop Fill |
Typically < 1.0% RSD,(5000 µl loop, 1000µl injection volume, draw/eject
speed 20 ml/min) |
| Carry-over | < 0.1% with rinse factor > 3 (see test method listing) |
| Area precision Complete Loop Fill | Typically < 0.5% RSD,(50 µl loop, overfill factor 3 or greater) |
| Area precision Partial Loop Fill |
Typically < 1.0% RSD,(5000 µl loop, 1000 µl injection volume, draw/eject
speed 20 ml/min) |
| Draw/eject speed | max. 50 ml/min (selectable in 1 µl/min steps) |
| Loop size | max. loop size 5 ml (selectable range: 5 µl – 50000 µl) |
| Sample viscosity range | 0.2 – 5 cp |
| Injection volume |
Selectable range: 0 – 50000 µl in 0.1 µl steps
Recommended range: 10 µl – 5000 µl in 1 µl steps |
|
Temperature controlled version
with G1330B |
Temperature range of sample compartment: 4 – 40 deg C |
| Sample capacity | 2 × well-plates (MTP) + additional 10 × 2 ml vials |
|
Supported sample
Plates |
Any combination of 2 of the following plates:
96 well plate (shallow/deep/conical) 54 x 2 ml vial plate 15 x 6 ml vial plate 27 x Eppendorf plate for Eppendorf tubes of 0.5 ml,1.5 ml or 2.0ml Maximum plate height with plate tray G2258-60001: 50 mm |
| Agilent 1200 Series Preparative Autosampler G2260A | |
|---|---|
| Type | Specification |
| Pressure | Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5800psi) |
| GLP features |
Early maintenance feedback (EMF), electronic records of
maintenance and errors |
| Communications |
Controller-area network (CAN). GPIB (IEEE-448), RS232C,
APG-remote standard, optional four external contact closures and BCD vial number output |
| Safety features |
Leak detection and safe leak handling, low voltages in maintenance
areas, error detection and display |
| Injection range |
0.1 – 900 µl in 0.1 µl increments (recommended 1 µl increments)
Up to 1800 µl with multiple draw (hardware modification required) Up to 5000 µl with multiple draw (hardware modification required) |
| Replicate injections | 1 – 99 from one vial |
| Precision |
Typically < 0.5% RSD of peak areas from 5 – 2000 µl, Typically
< 1% RSD of peak areas from 2000 – 5000 µl, Typically < 3% RSD of peak areas from 1 – 5 µl |
| Minimum sample volume |
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in
300 µl microvial |
| Sample viscosity range | 0.2 – 50 cp |
| Sample capacity |
100 × 2-ml vials in 1 tray
15 × 6-ml vials in ½ tray (Agilent vials only) |
| Injection cycle time | Typically 50 s, depending on draw speed and injection volume |
| Agilent 1200 Series G1315B Diode Array Detector | |
|---|---|
| Wavelength range | 190-950 nm |
| Light source | Deuterium and tungsten lamps |
| Detection type | 1024 – element photodiode array |
| Short term noise (ASTM) single & mult-wavelength | ± 1° x 10-5 AU at 254 and 750 nm |
| Drift | 2° x 10-3 AU / hr at 254 nm |
| Linear absorbance range | > 2 AU (upper limit) |
| Wavelength accuracy | ± 1 nm |
| Wavelength bunching | 1 – 400 nm |
| Diode width | < 1 nm |
| Flow cells |
Standard: 13µl volume 10 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-Micro: 5µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum Micro: 2µl volume, 3 mm cell path length and 120 bar (1760 psi) pressure maximum High Pressure: 1.7µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure max. 80 nano: 0.08µl volume, 100 mm cell path length and 50 bar (725 psi) pressure max. 500 nano: 0.5µl volume, 10 mm cell path length and 50 bar (725 psi) pressure max. |
| Analog outputs |
Recorder / integrator: 10 mV or 1 V, output range 0.001 – 2 AU, two outputs |
| Control and data evaluation | Agilent Chemstation for LC |
| Slit width | 1, 2, 4, 8, 16 nm |
| Communication types | CAN, GBIP, RS-232C, APG, Remote: ready, start, stop and shut down |
| Wavelength range | 190-950 nm |
| Light source | Deuterium and tungsten lamps |
| Detection type | 1024 – element photodiode array |
| Short term noise (ASTM) single & mult-wavelength | ± 1° x 10-5 AU at 254 and 750 nm |
| Drift | 2° x 10-3 AU / hr at 254 nm |
| Linear absorbance range | > 2 AU (upper limit) |
