This system includes:
- (1) Agilent 1290 G4220A Binary Pump
- (1) Agilent 1260 G4226A Autosampler
- (1) Agilent 1290 G1316C Thermostatted Column Compartment (TCC)
- (1) Agilent 1260 G1314F Variable Wavelength Detector (VWD)
Agilent 1290 G4220A Binary Pump
| Type | Specifications |
|---|---|
| Hydraulic system | Two dual pistons in series pumps with proprietary servo-controlled variable stroke design, power transmission by ball screws, smooth motion control of pistons for active damping. |
| Settable flow range | Setpoints 0.001—5 ml/min, in 0.001 ml/min increments. Executed in 300 pl/step increments |
| Flow precision | ≤0.07 % RSD or 0.005 min SD, whatever is greater (0.2—5.0 ml/min). Based on retention time at constant room temperature. |
| Flow accuracy | ±1 % or 10 μl/min, whatever is greater. Pumping degassed H2O at 10 MPa (100 bar) |
| Pressure operating range | Operating range up to 120 MPa (1200bar), up to 2ml/min, 80MPa (800 bar) at 5 ml/min. |
| Pressure pulsation | <1 % amplitude or < 0.5 MPa (5 bar), whatever is greater. At 1 ml/min water |
| Compressibility compensation | Automatic, pre-defined, based on mobile phase selection. |
| Gradient formation | High pressure binary mixing. |
| Delay volume | Jet Weaver V35: <45 μl Jet Weaver V100: <75 μl |
| Composition range | Settable range: 0 – 100 % |
| Composition precision | <0.15 % RSD, or 0.01 min SD, whatever is greater. 0.2 —5.0 ml/min; based on retention time at constant room temperature |
| Composition accuracy | ±0.35 % absolute (5 – 95 %, 0.2 – 5.0 ml/min) |
| Solvent selection valve | Default |
| Integrated degassing unit | Number of channels: 2Internal volume per channel: 1.5 mL |
| Control | Agilent ChemStation for LC (B.04.02 or above) EZChrom Elite (3.3.2 SP1 or above) OpenLAB (3.3.2 SP3) Masshunter (B.02.01 SP1 or above) |
| Local control | Agilent Instant Pilot (G4208A) (B.02.08 or above) |
| Communications | Controller-area network (CAN), RS232C, APG remote: ready, start, stop and shutdown signals, LAN |
| Safety and maintenance | Extensive diagnostics, error detection and display (through Agilent Lab Advisor), leak detection, safe leak handling, leak output signal for shutdown of the pumping system. Low voltage in major maintenance areas. |
| GLP features | Early maintenance feedback (EMF) for continuos tracking of instrument usage in terms of purge valve switches and volume of pumped mobile phase with pre-defined and user settable limits and feedback messages. Electronic records of maintenance and errors. |
| Housing | All materials recyclable. |
Agilent 1260 G7167A Multisampler
- Sample drawers are available in three heights to accommodate any combination of sample containers for maximum flexibility
- Shallow microplate drawers take a maximum load of 16 microplates and up to 6,144 samples for unmatched sample capacity
- Robotics move microplates and other sample containers from sample hotel to central workspace for seamless automation during sample processing steps and injections
- Dual-needle setup enables reduction of injection cycle times to mere seconds, virtually eliminating conventional wait times
- Dual-needle injection paths can be individually optimized for enhanced injection flexibility
- Multiwash capability flushes the outside surfaces of the injection needle outside with three solvents and uses seat backflush procedures to reduce carryover to less than 9 ppm
- Integrated sample thermostat, available as option or upgrade, provides cooling and heating from 4 to 40 °C
- Lights on each drawer indicate loading status, current activity, and accessibility for instant information
Agilent 1290 G1316C Thermostatted Column Compartment
- Temperature precision specification of ± 0.05 degrees for repeatable retention times and peak areas.
- Maximum application flexibility through Peltier cooling and heating for two independent temperature zones from 10°C below ambient temperature up to 100°C.
- Low dispersion – optimized for low internal volume contribution.
- Spacious column racks provide room for three 30 cm columns or several shorter columns.
- Ultra high pressure valves for many applications such as alternating column regeneration, sample preparation and dual or multi-column selection.
- Unique pull-out and Quick Change Valve design for simplistic plumbing and valve exchange.
- Clustering of up to three 1290 Infinity TCC modules for maximum flexibility for multi-method applications such as automated method development.
- Further features include a door-open sensor, improved thermal insulation, leak funnel and capillary guide.
Agilent 1260 G1314F Variable Wavelength Detector (VWD)
- Robust quantification of trace level components: Lowest baseline noise and drift results in lowest detection limits
- More analyte information per run with dual-wavelength capabilities
- High-resolution gain in fast LC at up to 120 Hz data acquisition rate
- Wide linear range (>2.5 AU upper limit) for reliable, simultaneous quantification of primary compounds, by-products, and impurities
- Efficient temperature control: Next-generation electronic temperature control (ETC) provides maximum baseline stability and practical sensitivity under fluctuating ambient temperature and humidity conditions
- Automatic wavelength verification provided using built-in holmium oxide filter
- Fast wavelength optimization with stop-flow wavelength scanning
- New levels of data traceability with radio frequency identification (RFID) technology for flow cells and lamps
- Maximum flexibility, compatibility, and investment protection with a range of eight analytical and preparative flow cells
- Continuous tracking of instrument usage: Early maintenance feedback (EMF) continuously tracks lamp burn time with user-defined limits and message types
- Extensive analytics, error detection, and displays using Agilent Lab Advisor software
