The VWD-3400 Variable Wavelength Detector is a high-quality instrument designed for HPLC analysis as part of a Thermo Fisher/ Dionex UltiMate 3000 system. The module was developed especially for HPLC analysis and can be used in numerous laboratory environments for routine analysis and sophisticated HPLC research tasks.
Specifications:
| Type | Specifications |
|---|---|
| Optical design | Dual-beam photometer (monochromator) Multiple wavelength UV/VIS detector |
| Light source | Deuterium lamp and tungsten lamp Temperature control for both lamps |
| Wavelength range | 190-900 nm The tungsten lamp is recommended for wavelengths > 600 nm. |
| Noise | Single wavelength: <± 3.5 μAU (typical <± 2,5 μAU): dry analytical flow cell, wavelength: 254 nm, time constant (according to ASTM): 1 second, only deuterium lamp turned on <± 5 μAU (typical): wet analytical flow cell, wavelength: 254 nm, time constant (according to ASTM): 1 second, only deuterium lamp turned on, flowing HPLC grade methanol at 1.0 mL/min <± 5 μAU (typical): dry analytical flow cell, wavelength: 520 nm, time constant (according to ASTM): 1 second, only tungsten lamp turned on <± 7.5 μAU (typical): wet analytical flow cell, wavelength: 520 nm, time constant (according to ASTM): 1 second, only tungsten lamp turned on, flowing HPLC grade methanol at 1.0 mL/min All values reached within 60 minutes of warm-up (typical). Multiple wavelength: <± 25 μAU (typical: <± 10 μAU): dry analytical flow cell, wavelengths: 254 nm and 280 nm, time constant: 2 seconds, data rate: 2 Hz, only deuterium lamp turned on <± 30 μAU (typical: <± 10 μAU): dry analytical flow cell, wavelengths: 450 nm and 520 nm, time constant: 2 seconds, data rate: 2 Hz, only tungsten lamp turned on <± 50 μAU (typical: <± 10 μAU): wet analytical flow cell, wavelengths: 450 nm and 520 nm, time constant: 2 seconds, data rate: 2 Hz, only tungsten lamp turned on, flowing HPLC grade methanol at 1.0 mL/min All values reached within 60 minutes of warm-up (typical). |
| Drift | < 1 x 10-4 AU/hour: dry analytical flow cell, wavelength: 254 nm, only deuterium lamp turned on, constant ambient conditions (temperature, humidity) Typical < 1 x 10-4 AU/hour: dry analytical flow cell, wavelength: 520 nm, only tungsten lamp turned on, constant ambient conditions (temperature, humidity) Typicial < 2 x 10-4 AU/hour after 60 minutes warm-up |
| Linearity | <5% at 2.5 AU caffeine, wavelength: 272 nm, based on ASTM (correlation coefficient > 99.97%, <3% RSD up to 2.5AU) <5% at 2.5 AU cobalt, wavelength: 520 nm (correlation coefficient > 99.95%, <3% RSD up to 2.5AU) |
| Wavelength calibration | Internal calibration with zero order and D-alpha line of the deuterium lamp |
| Wavelength verification | Internal verification with holmium oxide filter |
| Data collection rate | Single wavelength: Adjustable, up to 100 Hz (VWD-3400) or 10 Hz (VWD-3100) Multiple wavelengths: Adjustable, up to max. 5 Hz, depending on the number of wavelengths (2-4) and the distance between the wavelengths (in nm) |
| Wavelength accuracy | ± 1 nm (over detector lifetime) |
| Wavelength repeatability | ± 0.1 nm |
| Optical bandwidth | 6 nm at 254 nm |
| Flow cells | Analytical standard flow cell: Path length: 10 mm; cell volume: 11 μL; pressure limit: 120 bar (stainless steel), 50 bar (PEEK) Optional flow cells for nano, cap, micro, and semipreparative HPLC Identification of flow cell type and serial number via built-in ID chip |
| Control | All parameters and functions software controlled, USB 3 LEDs (Power, Connected, Status) for status monitoring |
| I/O interfaces | 4 digital input, 4 digital outputs |
| Analog output | 2 analog outputs available as an option via DAC plug-in module software selectable: absorbance, 20 bit resolution, 0-1V (full range) and 0-10 V (full range with adjustable mAU ranges). |
| Spectrum scanning | Yes, with different scan speeds |
| GLP features | In Chromeleon: Full support of automatic equipment qualification (AutoQ) and system performance monitoring. All system parameters are logged in the Audit Trail. |
| Safety feature | Power-up diagnostics of optics, cooling fans, motors, and electronics Leak sensor |
| Wetted parts | PEEK, quartz glass, stainless steel |
| Environmental conditions | Range of use: Indoor use Temperature: 10 °C to 35°C Air humidity: 80% relative humidity, non-condensing Overvoltage category: II Pollution degree: 2 |
| Dimensions (h × w × d) | 16 × 42 × 51 cm |
