The Flexar FX-UHPLC Autosampler is a powerful high-throughput UHPLC autosampler capable of operation at up to 18,000 psi. Comes standard with Peltier cooling, and a high-pressure injection valve for the most advanced UHPLC applications.
| Hardware Specifications | |
| Pressure limits | 18,000 psi (1,240 bar) |
| Injection modes | Fixed Loop: 5x sample overfill
Partial Fill: Variable injection volumes µL Pickup: Injections without any excess sample waste |
| Injection volume | Fixed Loop mode: 2 µL to 500 µL, depending on loop volume
Partial Fill mode: 0.1 µL to 2.45 mL (up to half loop volume) µL Pickup mode (no sample waste): 1 µL to 977 µL, depending on loop volume |
| Injection cycle time | As fast as 12 seconds in Partial Fill mode or 35 seconds in µL Pickup mode |
| Loop volumes | 2 µL to 5 mL, 10 µL standard |
| Sample/flush syringes | 100, 250, 500, 1000 and 2500 µL; 250 µL standard |
| Injection repeatability | Fixed loop mode:
0.3% RSD
Partial loop mode: 0.5% RSD for ≥5 µL injections 1.0% RSD for 1-4 µL injections
µL Pickup mode: 0.5% RSD for ≥5 µL injections 1.5% RSD for 1 µL injection |
| Sample carryover | <0.005% (caffeine), using programmable needle wash |
| Sample capacity | 2 x 96-well high (deep) and low (shallow) microtiter plates; 100 2 mL vials; 200 0.2 mL vials; 25 6 mL vials |
| Tray types/ microtiter plates supported | 100-vial tray: 2 mL vials (standard)
85-vial tray: 80 2 mL vials plus 5 6 mL vials 80-vial dilution tray: 80 2 mL vials plus 60 mL dilution tank 205-vial tray: 200 0.2 mL micro vials plus 5 2 mL vials 25-vial tray: 25 6 mL vials 2 x 96-well microtiter plate, high (deep) 2 x 96-well microtiter plate, low (shallow) *All the above trays and plates are supported by Peltier Cooling and Heating options. All trays and plates are supported by missing-vial detection. |
| Derivatization/serial dilution | Both automated pre-column derivatization and serial dilution are supported using liquid mixing. No additional hardware is required other than an optional sample tray if desired. Tray used must provide large enough vials or a dilution tank for the diluent and/or derivatization reagent(s). Supported in Chromera® version 4.1 or higher, TotalChrom® and Agilent® EZChrom® 3.3.2 |
| Needle height above vial well/plate | 2-6 mm above vial well bottom or above bottom edge of microtiter plate |
| Injections per vial/plate | Limited only by sample volume and injection mode |
| Wetted materials in contact w/sample | Wetted materials in contact w/sample (Biocompatible flow path not available) |
| Needle washes/ flushes | Programmable: up to 9 each of pre- and post-injection and between-method needle washes/flushes. All washes are followed by air drying of needle via built-in air compressor |
| Peltier options | Cooling-only capability standard; Heating options available |
| Peltier temperature range | Cooling-only: 4 °C to ambient -3 °C (will reach 4 °C ±2 °C with ambient temperature up to 25 °C at relative humidity of 80%).
Cooling/Heating: 4 °C to 40 °C (will reach 4 °C ±2 °C with Injection start synchronized to start pump program). |
| Pump piston injection synchronization | Injection timing synchronized to pump piston position to optimize retention time reproducibility.* |
| Second solvent flush | Supported in Chromera version 4.1 or higher |
*Available only with Flexar FX-20 UHPLC Pump
| Operating Specifications | |
| Output | Injection start, typically used to start pump program |
| Input | Ready or Inject input, depending on how configured |
| Audible noise level | LeAq <70 dB |
| Working temperature | 10 °C to 40 °C (indoor use only) |
| Storage temperature | -25 °C to 60 °C |
| Humidity | 20% to 80% RH, non-condensing |
| Safety and EMC compatibility | According to EC directives; CSA (UL) approved |
| Power requirements | 95-245 VAC, 50/60 Hz (±1%),
200 VA maximum power consumption |
| Viscosity range | 0.1-5 cP |
| Dimensions (HxWxD) | With Peltier cooling/heating option:
36 x 30 x 57.5 cm (14.2 x 11.8 x 22.6 in) Without Peltier cooling/heating option: 36 x 30 x 51 cm (14.2 x 11.8 x 20.1 in) |
| Weight | With Peltier cooling/heating option:
21.0 kg (46.2 lbs) Without Peltier cooling/heating option: 19.0 kg (41.8 lbs) |
| Maximum weight which can be stacked on top of autosampler | 65 kg (143 lbs) |
| Software, System Control and Communication Specifications | |
| Service Manager software | Autosampler setup and troubleshooting using dedicated user interface |
| CDS system environments supported | TotalChrom and Chromera |
| Interface for TotalChrom environment | RS232 via dotLINK |
| Interface for Chromera environment | RS232 |
