Waters GPC/SEC HPLC System
This system includes:
- (1) Waters Alliance 2695 Separations Module
- (1) Waters Alliance Column Heater
- (1) Waters 2414 Refractive Index Detector
- (1) Millennium 32 gel permeation chromatography/ size exclusion chromatography Software
- Waters 2424 Evaporative Light Scanning Detector (optional)
Waters Alliance 2695 Separations Module
| Number of solvents | One to four |
| Solvent conditioning | Vacuum degas, two (2) operating modes, four (4) chambers, ≈<500 uL internal volume per chamber> <500uL interval volume per chamber |
| Programmable flow rate range | 0.000 and 0.010 to 10.000 mL/min in 0.001-mL/min. increments |
| Typical operating flow rate range | 0.050 to 5.000 mL/min in 0.001-mL/min increments |
| Compressibility compensation | Automatic and continuous |
| Effective system delay volume | <650 µL, independent of backpressure @ 1 mL/min |
| Plunger seal wash | Integra, active, programmable |
| Gradient profiles | Eleven (11) gradient curves [including linear, step (2 curves), concave (4 curves), and convex (4 curves)] |
| Dry prime/wet prime | Automatic, front panel control |
| Flow ramping | Time (0.01 to 30.00 min in 0.01-min increments) to reach maximum flow rate |
| Maximum operating pressure | 5000 psi (345 bar) (0.010 to 3.000 mL/min) Programmable upper and lower limits. Pressure fall-off at >3.000 mL/min. |
| Pressure ripple | ≤2.5% (1 mL/min, degassed methanol, at 1700 psi backpressure) |
| Composition accuracy | ±0.5% absolute, independent of backpressure (Proportioning Valve Pair Test [degassed methanol:methanol/propylparaben, 2 mL/min, 254 nm]) |
| Composition precision | ≤0.15% RSD or ≤0.02 min SD, whichever is greater, based on retention time. (Degassed methanol:water 60:40 Dial-a-Mix, 1 mL/min, six replicates, phenone mix, 254 nm.) |
| Flow precision | ≤0.075% RSD or ≤0.02 min SD, based on retention time (N = 6) or volumetric measures (0.200 to 5.000 mL/min), isocratic premix |
| Flow accuracy | ±1% or 10 µL/min, whichever is greater, (0.200 to 5.000 mL/ min), degassed methanol, at 600 psi backpressure |
Waters 2414 Refractive Index Detector
- Active nebulizer heating and cooling for method optimization and rapid stabilization
- 60° scatter angle detection to minimize stray light and optics polarization for long-term optimal sensitivity
- Optimal energy output for improved bench-to-bench repeatability
- Precise temperature control in the nebulization, evaporation, and detection stages, maintaining low-dispersion characteristics for improved resolution
- Independent optimization of sampling rates and filter time constants gives you the ability to tune your detector to your separation goals
- Nanogram-level detection limits
- Open-access-ready with accelerated nebulizer stabilization
- Sampling rates up to 80 points/s
- Reproducible results and long lamp lifetimes
- Mass response independent of analyte’s optical properties
- Improved detection for semi-volatile compounds
- Simple user maintenance with front mounted snap-in nebulizer and lamp
- Small, stackable footprint, minimizing laboratory space requirements
- Low dispersion volume for high-speed GPC and narrowbore column chromatography from 0.1 to 10 mL/min
- Thermally isolated optics bench for temperature stability and performance reproducibility
- Save solvent and maintain stability with convenient recycle valve
- Diagnostics and simplified design and control options for easy maintenance and everyday use
- Circuitry and software in the detector’s thermally isolated optics bench provide a sensitive, stable signal response to changes in refractive index independent of ambient temperature changes
- Auto-zero, auto-purge, and LED-displayed diagnostic programming enable unattended operation and to simplify methods development
| RI Range | 1.00 to 1.75 RIU |
| Measurement Range | 5 × 10–4 RIU, 7 × 10–9 RIU |
| Flow Rate | 0.1 to 10 mL/min |
| Noise | ± 1.5 × 10–9 RIU, in RIU mode (Hamming filter TC = 2, ambient temperatures 23 to 25°C (± 2 °C/hr), 1 mL/min. 100% water. ± 3.0 × 10–9 RIU, in 410 mode (RC filter TC = 1, ambient temperatures 23 to 25°C (± 2 °C/hr), 1 mL/min. 100% water. |
| Drift | 2.0 × 10–7 RIU/hr |
| RIU-FS Settings | (RIU mode) 1.0 × 10–6 to 5.0 × 10–4 RIU |
| Sensitivity Settings | (410 mode) 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024 |
| Time Constant Filter Settings | 0.0 to 5.0 seconds (Hamming) 0.0 to 10.0 (RC) 0.2, 1, 3, 10 seconds (RC under software data control) |
| Light Source | Light emitting diode (LED) |
| Flow Cell | Fused quartz |
| Cell Volume | 10 µL |
| Pressure Limit | 100 psi |
| Fluidic Path Materials | 316 stainless steel, PTFE, peek, quartz |
